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KMID : 0894520070110030219
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Development & Reproduction
2007 Volume.11 No. 3 p.219 ~ p.226
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Depletion Effects of Chick Germinal Crescent’s Primordial Germ Cells by Heat Activated Busulfan Injection
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Jung Dong-Kee
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Abstract
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This study was conducted to identify optimistic primordial germ cells’(PGCs) migration activity using heat activated busulfan treatment for the increasing germline chimerism. Donar PGCs viability tests of important conditions for useful germ line chimerism indicated approximately 70～80% viability was time dependent. Transplantation experiments of PGCs into recipient embryos after busulfun treatment, showed the treatment group having 23.5% viability. By comparison, the control group showed 4.8% viability. The 96 hour treatment group and the 118 hour treatment group of the cultured PGCs showed high migration activity. Generally, the transplantation method would consider morphological and physiological characteristics before transplantation. In the present study, the effect of busulfan on migration activity showed viability highest at 53.4% after 48-hour incubation time. However, a previous study showed the best condition for transplantation time to be prior to the 48-hour incubation period, when the chicken embryo does not yet have a developed blood vessel system. In conclusion, an important condition for the production of a transgenic chicken is that most donor PGCs migrate into the recipient embryo without any inhibitory factors. The present results suggest, perhaps by using this modified method of transplantation, it can produce a more efficient chimeric germ line, transgenic chicken.
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KEYWORD
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Primordial germ cells, Migration activity, Busulfan, Germline chimeric chicken, Transgenic chicken
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